Lab Report on Diffusion and Osmosis Our objective is to figure diffusion and osmosis. Diffusion is the process whereby particles of liquids, gases, or solids intermingle as the result of their spontaneous movement caused by thermal agitation and in dissolved substances move from a region of higher to one of lower concentration. Osmosis is a process of absorption or diffusion suggestive of the flow of osmotic action. In our experiment in lab class, we did a procedure to observe osmosis along a free energy gradient. It was to determine if the experiment was hypotonic, hypertonic, or isotonic. Our second experiment was to observe diffusion across a differentially permeable membrane. It is to figure out movement and reaction of sodium hydroxide and phenolphthalein through a permeable membrane. In addition, we also figure the movements and reaction of iodine and starch in a different beaker.
In our experiment to observe osmosis along a free energy gradient, we would need to gather all these supplies.
* We would first obtain eight pieces of strings and four pieces of dialysis tubing that is pre soaked about fifteen cm long. Then we would seal one end tightly by folding and tying.
* We would have to leave one end open to fill it up with the different percentages of sucrose.
* In the first dialysis tube (bag A), we would insert ten mL of one percent sucrose. Bag B we would also insert ten mL of one percent sucrose. Bag C would be different it contains ten mL of twenty-five percent sucrose. Bag D has a higher percentage of sucrose of fifty percent also ten mL. We also label these bags by writing the letters A-D and place them in the bag.
* After the solution sucrose, which is a disaccharide composed of glucose and fructose. Is inserted we would tie the ends off.
* Then we would weight the bag and record it in a chart before we put it in the beaker containing sucrose solution.