THE EFFECT OF TEMPERATURE ON ENZYME ACTIVITY
• The main aim of this experiment is to see if temperature has any effect on the enzyme activity.
The hypothesis I am going to use is: -
• Hydrogen peroxide will form oxygen gas and water when it is broken down.
• More gas will be produced as the temperature increases. This is because the particles will gain more kinetic energy hence will be moving faster, leading to a successful collision.
• I can predict that if the temperature is above the normal body temperature (370C), the amount of gas being produce will eventually decrease and the enzyme will denature as enzymes are known to work best at body temperatures.
• Water bath
• Crushed ice
• Screw clip
• Stop clock
• Delivery tube with bungs to fit the test tubes/boiling tubes
• Hydrogen peroxide
• Soaked peas
• 10cm3 syringe barrel
• Test tubes or boiling tubes
• 10ml Measuring cylinders
• Distilled water
In this experiment the DEPENDENT VARIABLE is going to be the temperature while the INDEPENDENT VARIABLE is going to be the amount of oxygen gas collected.
I will use 4 crushed peas of the same/similar size for all the investigation. I will also make sure that I use 4cm3 of hydrogen peroxide and 2cm3 of distilled water to make it a fair test.
• To wear safety goggles to protect your eyes from the vapour from the hydrogen peroxide as it is an irritant.
• To handle the glassware with care making sure nothing will break as they are very fragile.
• Crush 4 peas of the similar size and add 2cm3 of water to it and pour the crushed peas into the boiling tube.
• Put the boiling tube in the water bath with crushed ice as shown in the diagram below.
• Then open the cock of the boiling tube and add the 4cm3 of the hydrogen peroxide to the...