Genetics of Sordaria Fimicola

Genetics of Sordaria Fimicola

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  • Date Submitted: 03/12/2010 6:46 PM
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GENETICS OF SORDARZA FZ-MICOLA. VII. GENE CONVERSION AT THE G LOCUS I N INTERALLELIC CROSSES
Y. KITAN1 AND L. S. OLIVE
Department of Botany, University of North Carolina, Chapel Hill, N . C. 27514
Received October 9,1968

HE preceding paper in this series (KITANI and OLIVE 1967) dealt with gene Tconversion in crosses between g heteroalleles and wild type. The investigation of conversion at this locus is continued in the present study of crosses between the various heteroallelic mutants.
MATERIALS A N D METHODS

The following heteroallelic mutants of the g locus were studied: g, (gray-spored), the original hyaline-spored isolates h,, h,, and h,, and the derivative hyaline-spored mutants h,, and hZb. The origin, nature, and complementation relationships of these mutants have been described (KITANI and OLIVE 1967). The linked morphological markers have also been described elsewhere (EL-ANI, OLIVE and KITANI1961), as has their behavior in relationship to conversion at the g locus (KITANI,OLIVEand EL-ANI 1962; KITANI1963; KITANIand OLIVE1967). Linkage relationships of the morphological markers to the g locus are shown in Figure 1. An unlinked marker i, which imparts a slight indigo color to the spore wall, was used in most crosses to distinguish certain types of cmversion asci from those having spindle overlap (“spore slippage”). mat h sp h, cor The crosses studied were: -, where h represents h,, h,,, h,, or hlb, sp g , cor mat g, mat g,h, cor mat g,h, cor , and

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Aberrant asci were detected by direct microscopic examination, after which they were dissected to determine the genotypes of all germinable spcres. Normal appearing 4 gray:4 hyaline asci resulting from conversion are visually indistinguishable from the background population of 4:4 asci and are therefore excluded from the dissections. This includes normal appearing 4:4 conversion asci containing one or two double...