Investigation of action of saliva and hydrochloric acid in two carbohydrate solutions.
To investigate the action of saliva and hydrochloric acid in two carbohydrate solutions.
Apparatus and Equipments
10 Boiling tubes, Metal test tube racks, Beaker, Graduated plastic dropper, White tile, Water bath, ~ 37 degree celcius and Water bath, ~ 95 degree celcius.
Carbohydrate solution A, Carbohydrate solution B, Benedict’s solution, 3 M of Hydrochloric acid, 3 M of sodium hydroxide and Iodine solution.
1. Two boiling tubes containing 1 ml of solution A and 1 ml of solution B was prepared respectively. 1 ml of Benedict’s solution was added into each tube. Both of tubes were heated together in the temperature of ~ 95 degree celcius water bath for about +- 4 minutes. The results were recorded in Table 1.
2. A few drops of fresh solution A and B was added separately spaced on a white tile. On each solution, 1 to 2 drops of iodine solution was added. Observations were recorded in Table 1.
3. 2 ml of solution B was pipetted into each of four boiling tubes. Tubes 1, 2, 3 and 4 were labelled respectively near the mouth of tube. Group name was labelled on respectively tubes.
4. Tubes 1 and 2 were placed in a water bath of temperature of ~ 37 degree celcius about +- 3 minutes.
5. A small beaker was salivated till it reaches about 5 ml.
6. Step 6 and 7 is to be done approximately at the same time. 4ml of the saliva was measured out to prepared in step 4 and 2 ml was pipetted each into tubes 1 and 4. The contents of the tubes were shaken well to ensure thorough mixing.
7. 4 ml of HCL was measure out and 2 ml was pipetted each into tubes 2 and 3.
8. Tubes 1, 2, 3 and 4 were incubated in their respective temperatures as shown in Table 2 for 35 minutes from the initial moment.
9. 4 more new boiling tubes were labelled as follows: 1’, 2’, 3’ and 4’.
10. After 5 minutes of incubation from the initial moment of tubes 1 and...