MAINTAINING A BALANCE EXPERIMENTS
DOT POINT 1: MOST ORGANISMS ARE ACTIVE IN A LIMITED TEMPERATURE RANGE_______________________
DOT POINT CAPITAL A:
• Identify data sources, plan, choose equipment or resources and perform a first-hand investigation to test the effects of:
* Increased Temperature - Change in pH -Change in Substrate
* Change in substrate concentration on the activity of the named enzymes.
∆ EXPERIMENT: ENZYME ACTIVITY VS SUBSTRATE CONCENTRATION
* Aim: To investigate the effect of substrate concentration in the rate of enzyme activity.
* Hypothesis: Enzyme activity increases with increasing substrate concentration
1. Collect apron, safety glasses, gloves and put on. Lay out newspaper in the bench
2. Cut two 2-3 mm size (rice size) pieces of liver using paper, a cutting board and play in separate identical test tubes, labelled A and B.
3. Measure 1 mL of Hydrogen Peroxide using a measuring cylinder and add to a new test tube and label C. Acclimatise to room temperature for 2 minutes and add this to the test tube A. Observe the rate of reaction as + (low), ++ (medium), +++ (high) and measure the height of the bubbles using a mm line ruler. Repeat this for the 6% hydrogen peroxide, labelling the test tube D and recording it in B.
4. Repeat steps 1-3 twice more and compare results for consistency. Average the three sets of height data and record it in a table.
5. Rinse the test tubes out, catching the liber in a sieve and disposing of it in the bin, along with the newspaper and gloves. Return the equipment when instructed and wipe down the bench with disinfectant.
* Results: Table 1: Enzyme activity for 3% and 6% substrate concentrations
* Discussion: The reaction rate for the 3% peroxide was much lower than the 6% peroxide. This is because the higher the substrate concentration, the greater the rate of enzyme reaction, until all...