A Research Project Proposal
On
Cloning, expression, purification and characterization of bromodomain containing protein in Plasmodium falciparum
Submitted to
THE DIRECTOR
Defence Research and Development Establishment,
Gwalior
Submitted by:
Dr. Ritu Gill
(Principal Investigator)
Centre for Biotechnology
Maharshi Dayanand University,
Rohtak – 124001
Haryana
1. Submitted by : Ritu Gill (Principal Investigator)
Lecturer
Centre for Biotechnology,
Maharshi Dayanand University,
Rohtak-124001, Haryana
2. Particulars of preliminary examination/feasibility project study on which based
Malaria is one of the word’s biggest health problems. P. falciparum is one of four Plasmodium species that infects human, is responsible for most malaria related deaths. Malaria parasites have a complicated life cycle with two different hosts - a vertebrate and an invertebrate. Each of these distinct environments compels the pathogen to exhibit differential gene expression during its life cycle. Chromatin remodeling is likely to play key roles in parasite gene regulation. However, detailed mechanisms that control parasite gene expression are sparse. The bromodomain first reported in the Drosophila protein brahma represents an extensive family of evolutionarily conserved 110 aminoacids protein modules. Bromodomains are generally found in proteins that regulate chromatin structure and gene expression, such as histone acetyltransferases and the ATPase component of certain nucleosomes-remodeling complexes. While it has been long suggested from yeast genetic studies that bromodomains play an important role in chromatin remodeling, their specific biological functions only began to emerge after the discovery that bromodomains function as acetyl-lysine binding domains. However, to...