The Role of NNAT in Determining the Pluripotent States of Human Induced Pluripotent Stem Cells
Name: Simam Thanka
Project report in partial fulfilment for the degree of MSc in Neuroscience August 2012
Contents
ABSTRACT…...............................................................................................................................2
1. INTRODUCTION
1.1. The Origin of Pluripotent Stem Cells..........................................................................3
1.2. Mouse Embryonic Stem Cells................................................................................... 4
1.3. Human Embryonic Stem Cells....................................................................................5
1.4. Epiblast Stem Cells.....................................................................................................6
1.5. Benefits of ES Cells: Genetic Modification.................................................................6
1.6. Benefits of Human ES Cells.........................................................................................7
1.7. Induced Pluripotent Stem Cells...................................................................................8
1.8. Pluripotent States.......................................................................................................9
1.9. Metastable States of Pluripotency and Conversion...................................................10
1.10. Neuronatin: A Putative Regulator of Pluripotent States..........................................14
1.11. Hypothesis and Aims................................................................................................16
2. MATERIALS AND METHODS
2.1. Culturing human iPSCs................................................................................................17
2.2. Converting hiPSCs from ‘primed’ to ‘naive’...