Tosh Foerster
Catalase Lab
2. The measurement of the foam indicates the activity of the enzyme because the amount of foam shows how much of the hydrogen peroxide is decomposed into water and gas. The enzymes job is to break down the hydrogen peroxide and the amount that it breaks it down is shown in the foam.
4. The effect of temperature on the enzyme is directly related to the amount of hydrogen peroxide decomposed. Seeing as how when the water is 10oC (50oF) or 80oC (176oF) the foam does not go up nearly as much as when it is 30oC (86oF) which is the closest to our normal body temperature. This must be why our body temp. is 98oF.
5. Enzyme activity is destroyed by denaturation because the enzyme has a specific shape or active sight that fits to only one type of substrate. Without the active sight the substrates can never be broken apart therefore decreasing enzyme activity.
6. Having a high body temperature over 105oF can be very dangerous because the enzymes will become denatured and won’t be able to break apart harmful substrates like hydrogen peroxide into more useful compounds like water and oxygen.
9. pH does have an effect on enzyme activity. The more acidic or basic the pH is the less effective the enzymes are.
10. Because of the fact that the enzymes prefer the homeostatic pH of 7 cells try to maintain that pH level so that the enzymes do not become denatured.
Tosh Foerster
Catalase Lab
2. The measurement of the foam indicates the activity of the enzyme because the amount of foam shows how much of the hydrogen peroxide is decomposed into water and gas. The enzymes job is to break down the hydrogen peroxide and the amount that it breaks it down is shown in the foam.
4. The effect of temperature on the enzyme is directly related to the amount of hydrogen peroxide decomposed. Seeing as how when the water is 10oC (50oF) or 80oC (176oF) the foam does not go up nearly as much as when it is 30oC (86oF) which is the closest to our normal body...