Glucose Estimation

Glucose Estimation

Estimation of Glucose in solution Using Glucose oxidase
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Institution

Estimation of Glucose in solution Using Glucose oxidase
Aims and Objectives
The objectives of this practical were:
The practical is meant to enable the student to understand the importance of measuring glucose levels in blood, food, urine and CFS. This practical would enable the learner understand the major principles of major principles of enzymatic estimation of glucose. The student should be able to apply the standard curve in determining the levels of glucose in different substances and also demonstrate the specificity of GOD to glucose.
Introduction
Glucose is one of the simple sugars which are usually a primary source of energy in the human body cells; the food that we consume contains glucose which is absorbed in the body for provision of energy after it is absorbed in the body by the intestines. The level of glucose in human body ranges from 70 to 100 mg/dl and is regulated by insulin and glucagon hormones in the body (McCurry, 1988).
There two main methods used in determining the levels of glucose in the body which include; enzymatic and non-enzymatic methods. When enzymatic methods are used, the presence of all reducing sugars in the body fluid is indicated, and when the non-enzymatic methods are used, the person only the presence of glucose in the fluid is indicated. This implies that non-enzymatic methods are more accurate as compared to enzymatic method since they only give the levels of glucose in the sample being tested rather the presence of reducing sugars as is the case with enzymatic methods (McCurry, 1988).
Non-enzymatic assays methods used in the measurement of glucose levels in different substances are; Benedict’s test which uses the benedict’s solution or copper sulphate solution to test the levels of glucose in fluids and mainly to estimate glucose levels in urine and blood. The Fehling’s method, this method uses Fehling’s solutions which like benedict’s...

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