Introduction
Trimyristin is a triglyceride, which is found in animal fats and vegetable oils with the following structure:
Trimyristin in non-polar hence it is immiscible with aqueous medium; meanwhile, it dissolves in organic compound such as hot acetone.
In this experiment, trimyristin was isolated from nutmeg seed by solvent extraction. The isolated sample was then purified by crystallization.
Objective
To isolate a natural product from a mixture and purify by recrystallization
Procedure
15g of ground nutmeg seed, 40ml of acetone and five antibumping granules were placed into a 100ml round bottom flask. The glass joints of the round bottom flask and the outer joint of the reflux condenser were lubricated by Vaseline to prevent freezing and knocking of the joints. The content were allowed to reflux for 15 minutes. A stemless funnel was preheated at around 80℃, fitted with a fluted filter paper, the refluxed content was then poured through the funnel for filtration. Residue was rinsed with 15ml acetone for extraction. Extracts were allowed to stand in an ice bath for recrystallization to complete, suction filtration was carried out to get the solid materials.
Experiment 4
Introduction
Recrystallization is carried out to increase the purity of the sample.
Infrared spectroscopy shows the infrared spectrum of the sample. Different functional groups with different bond types have different vibration frequencies due to stretching and bending, hence part of the frequencies of the infrared will be absorbed when passing through the sample, while part of the frequencies can be transmitted, absorption peaks are present and this is a useful technique to identify the functional groups present.
Objective
To purify the sample and carry out infrared spectroscopy to identify the functional groups present.
Procedure
Sample contained in the previous experiment was dissolved by 30ml acetone, accompanied by very small amount of...