Austin Chao
1/20/10
Ap Bio Lab
Transformation Lab
Purpose: The purpose of this lab was to add new traits to the e.coli bacteria and then see if they took on these traits in a new generation of offspring’s.
Background: Transformation is the genetic altering of a cell, this was an accomplished in this lab by adding the calcium chloride to help break up the cell membrane of the e.coli bacteria and then using a heat sink in order to make the DNA of the bacteria move and break up the cell membrane even more. A plasmid is a round shape of DNA found in bacteria, the traits we used with our plasmid is an antibiotic resistance. The traits got into the plasmids by being part a DNA sequence that formed the plasmid. We will know if the transformation is successful if we see the traits expressed in our positive gene control plates.
Data Results:
LB+ AMP(-) AMP(+) AMP/XGAL(-) AMP/XGAL(+)
Analysis: In the LB+ plate we were testing to see if our bacteria could grow at all, in the AMP(-) plate we were seeing if the bacteria with no plasmid of anti-bacterial resistance would die to the disease, next in the AMP(+) we were testing if our bacteria really did transform and receive the anti-bacterial resistance trait, then in the AMP/XGAL(-) we were testing one if the bacteria really died to the disease, if it didn’t did it show blue bacteria and finally in the AMP/XGAL(+) we were testing two things if the anti-bacterial disease resistance trait worked and if the XGAL really did work and make the bacteria a blue color. Our transformation efficiency for the AMP(+) plate was 6.123 squared which was received by dividing the number of bacteria colonies we received which was 6 by the mass of the pAMP cell suspension that was spread of the plate which is .0098mg which lead to 6/.0098 = 6.123 , our transformation efficiently in the AMP/XGAL(+) plate was a bit higher we divided 8/.0098 and received a efficiently of 8.163...