Biological active peptide research:
The DA201 - C type of macroporous adsorption resin for purification and debitterizing rapeseed protein hydrolysate.Dynamic adsorption and desorption experiments show that the rapeseed protein hydrolysate on pH value after adjustment for 4 column, use the same pH value of deionized water after being acquitted, again with 85% ethanol solvent desorption, protein recovery rate was 66.7%.The palm oil mill (http://www.palmmill.com)crude peptide (crude rapeseed peptides, CRPs) taste is more bitter, sugar and ash content decreased significantly, protein content increased from 47.0% before purification to 73.5% after purification, antinutritional factors glucosinolates and phytic acid has not been checked out.For points away from the bitter components of CRPs, after water desalination with different concentrations of ethanol solution (25%, 55% and 85%) elution step by step, three components of rapeseed peptides can be obtained: RP25, RP55 and RP85, its ratio of 64 ~ 66%, respectively, 32% ~ 32% and 5 ~ 29.RP25 the most shallow color, protein content was highest (81.04%), tannin content is the lowest (0.91%), no bitter, therefore can be used as an ideal food ingredients.Rapeseed peptides were studied using in vitro models respectively of antioxidant and antithrombotic activity.The kernel recycle station (http://www.palmmill.com/Products/prod/) results show that the rapeseed peptide has broad spectrum of scavenging free radicals can force activity and inhibition of lipid peroxidation, and increase with the increase of the concentration.RP25, RP55 and CRPs remove DPPH · ED50, 41, 499, and 72 mu, respectiely, in g/mL;RP55 CRPs and remove ED50 of O2 - · were 0.91 and 1.70 mg/mL, the removal ability were significantly (p < 0.05) than RP25;Under the same concentration RP25 ability to remove · OH significantly (p < 0.05) than RP55 and CRPs and RP25 and RP55 ED50 of · OH were 2.53 and 6.79 mg/mL.RP55 and ED50 of CRPs inhibition of lipid...