Abstract: The purpose of the enzyme reaction lab was to see how enzymes reacted when effected by different acids. To start this procedure, we labeled four test tubes A, B, C, and D. Second, we poured .5 mL into all four test tubes. Then, we poured .5 mL of pH 2 into test tube B, .5 mL of pH 7 into test tube C, and .5 mL of pH 9 into test tube D. Then we let the tubes sit for about three minutes to mix together. After they sat, we added 1 mL of Benedicts into all four test tubes and put them into a hot water bath for ten minutes to let them react. After the ten minutes was up, we took them out of the bath and observed the changes. As we recorded the results, we noted that the reaction worked better in a more acidic environment. The reaction worked the best with the pH of 2 (most acidic), turning a musty orange. It worked the least in pH 9 (the highest base), staying blue. In conclusion, in order for enzymes to react properly, they need to be mixed with an acidic pH.
Background: The purpose of this lab was to observe how enzymes reacted in different pH levels. Worthington Biochemical Corporation did this same experiment and got a little bit different results. They observed that the best amylase reaction happened at a neutral pH of 6.7-7.0. But in our experiment we noted that the amylase with pH 7 only took on a small change in hue. I think that if we let it sit in the hot water bath for more time, then we would have noticed a more significant change.
Hypothesis: If amylase is combined with different pH’s, then it will only react with the more acidic pH’s.
4 test tubes
Hot water bath
Test tube rack
Test tube clamp
1. Prepare test tube rack holding four test tubes labeled A, B, C, and D
2. Pour .5 mL of amylase into test tubes A, B, C, and D
3. Pour .5 mL of pH 2 into test tube B
4. Pour .5 mL of pH 7 into test...