On Polysulphide Reductase Definition Introduction Function of polysulphide reductase The ability to reduce sulfur using or organic substrates as electron donors is widespread among thermophiles Some of the organisms are able to use and as the sole energy source. These organisms must couple sulfur reduction to ADP phosphorylation. Elemental sulfur, while having little solubility, is readily converted to polysulfide in aqueous solutions containing sulfide. The sulfur ring is cleaved by a nucleophilic attack of the sulfide, forming a linear polysulfide, as shown in the equation: {draw:frame} It should be noted that in addition to this , appears to have another sulfur/polysulfide reductase, as mutants lacking this enzyme were still able to grow by the reduction of either elemental sulfur or polysulfides, although the growth yield was about 25% of that obtained by the wild type Materials and methods _Enyzme isolation-_ _Enzyme _activites Molybdenum and protein determination _Extraction of the carboxamidomethyl derivatives_ their absorption spectra. _Hydrolysis of the carboxamidomethyl derivatives_ Results {draw:frame} _Polysulphide _reductase Discussion HS- --+ S + H + + 2 e- (a) HCO~ --+ CO 2 + H + + 2 e- (b) -S, - S- + H + + 2 e- ~ S 2- + HS- (c)fjoi CKN DWKnL KSK LCN SLD MLC SKLDM FKL MKF C M L D MLK CM DS KM FCL KM DCML SD,C;,DS' ;SLDX MEK NF CK JD NC LML ;D,M SM, MX KJSD EM LCM XLSD MCKSMDCX,:
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