Protease

Protease

  • Submitted By: Chelo
  • Date Submitted: 10/16/2008 12:19 PM
  • Category: Science
  • Words: 285
  • Page: 2
  • Views: 779

Study Questions and Analysis Part 1 The two polynucleotide chains that are coiled around each other to form the rigid double-helix do not change forms when the cold alcohol is added to the solution. The DNA remained thick, fairly easy to break and as long fibers that could be spoiled onto a glass rod. When alcohol is added to the denatured DNA it precipitates as thin, very fibrous strands that are very easy to break. This precipitate is different from that of the native DNA because when just adding the cool alcohol the temperatures stayed the same, therefore keeping the DNA durable. Whereas with the single-stranded DNA, denatured, is not supported by its other half, therefore making it susceptible to breaking easier. The action of DNAse I on DNA is that it turns the intact DNA into little fragments. In our experiment we noticed the strands that were able to be coiled around our rod were the intact ones and that the dissolved and chopped up strands staying in our alcohol making the solution cloudy. Study Questions and Analysis Part 2 The morphology of the isolated nucleus differs from that of the intact thymus cells by the nuclei suspension and the thymus cells were separate individual cells but looked string like. The nuclear suspension from start was very cloudy and heavily concentrated. After adding the SDS solution the nuclear suspension turned clear and into a somewhat gel like formation. Thymus tissue is ideal for DNA distraction because the small amount of cytoplasm around the nucleus makes it easier to get to and there will be more in a given space. Lab Report The Effects of DNAse, Denaturing of Structure and Cell Fractionation Introduction

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