By: Casey Collins 10-30-08 Section 12 Scientific Question Can photosynthesis occur without chlorophyll? Hypothesis Experiment 2 – Chlorophyll is necessary for photosynthesis to occur. Prediction Experiment 2 – If chlorophyll is present with photosynthesis, then starch will be present throughout the leaf. Introduction Photosynthesis consist of these two reactions (photochemical and biochemical) occur in the chloroplast of a plant cell. In the chloroplast, the photochemical (light) reactions convert light-energy to chemical energy captured in ATP and NADPH. The biochemical (dark) reactions use the ATP and NADPH produced by the photochemical reactions to reduce CO2 to sugars. Photochemical reactions occur on thylakoid membranes (inner membrane of chloroplast where light-reaction chemicals are embedded), as the biochemical reactions occur in the stroma (the matrix surrounding the granum in the inner membrane of the chloroplast; stacks of thylakoids are a granum) as said before by (Brooker, Stiling, Widmaier, & Graham, 2008) Materials and Methods Experiment 2 – Remove a leaf from a Geranium plant that has been illuminated for several hours. After immersing the leaf in boiling water for 1 minute, bleach the pigments from the leaf by boiling the leaf in methanol for 3-5 minutes. Boiling the leaf will remove pigments so that you can see the color changes of the iodine starch test. Place the leaf in a Petri-dish containing a small amount of water, and then add five to eight drops of iodine. Observe any color changes in the leaf. Repeat the experiment with a Geranium leaf that has been kept in dark, a Begonia leaf kept in light, and an Oxalis leaf kept in light. Results Table 1.0 Describing the Color Change in an Acidic Solution When Elodea is Added or Left Out Table 2.0 Describing the Color Change of a Certain Leaf Being Stained with Iodine Discussion Some suggested furthering the experiments investigation would be to test on more acidic and basic...