Aseptic Technique

Aseptic Technique

  • Submitted By: gxiaosan
  • Date Submitted: 10/05/2013 12:45 PM
  • Category: Science
  • Words: 475
  • Page: 2
  • Views: 114


Escherichia coli plate culture
Sterile nutrient agar plate
Inoculating loop
Tape and sharpie


1. Labeled on the bottom of sterile agar plate with student name, date and name of the microbe, which was Escherichia coli. Divided the plate into three sections by drawing lines on the bottom of the sterile agar plate.
2. Turned on the incinerator and let it heat up.
3. Inserted the inoculating loop into the incinerator for a few seconds to sterilize the loop. Took the inoculating loop out of the incinerator and let it cool for a few seconds while holding the inoculating loop handle in hand.
4. Held the cultured plate in the other hand and lifted the lid slightly like a clamshell. Touched the loop to an area on the cultured Escherichia coli plate where there were no bacteria growth to make sure the loop was cool. Then touched the growth on the cultured plate with the loop to collect a small Escherichia coli sample. Removed the loop and closed the lid of the cultured plate.
5. With the transfer loop in hand, opened the lid of the steri
Laboratory Result

1. There were about a dozen of colonies formed on the nutrient plate. Most of the colonies were circular in shape, raised in elevation, and had entire margin except a couple of the over lapping colonies. The surfaces of the colonies were smooth. All of the colonies were opaque and glistening. Only two colors of the colonies were observed, which were white and golden yellow.
2. The nutrient broth of the tube appeared a bit darker and more yellowish and flocculent. There was little sediment observed on the bottom of the tube. There were no ring or pellicle observed.


Sediment was observed on the bottom of the tube and flocculent substance was presented in the nutrient broth, which indicated there was bacteria growth in the nutrient broth tube. To know what kind of bacteria, a microscope slide observation was needed.
By observing...

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